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Objective To investigate the characteristics of dominant bacteria of colonic lavage fluid in patients with diarrhea-predominant irritable bowel syndrome (IBS).Methods Forty-six patients with diarrhea-predominant IBS (observation group) and 46 patients with abdominal pain or discomfort who was excluded IBS (control group) from January 2016 to January 2019 in Department of Gastroenterology,Shenzhen Longhua District People's Hospital were selected.During colonoscopy,2 pieces of descending colonic mucosal tissues were taken by forceps,then 4 ml of colonic lavage fluid was collected from the mucosa tissues to extract total DNA,and 10 dominant bacteria were detected by realtime fluorescence quantitative polymerase chain reaction (including Bacteroides,Bacteriodes prevotella,Bifidobacterium,Clostridium,Enteropathogenic Escherichia coli,Dialister pneumosintes,Firmicutes,Faecalibacterium prausnitzii,Salmonella,Lactobacillus,and data were selected for logarithm).The correlation of dominant bacteria between colonic mucosal tissues and colonic lavage fluid was analyzed by Spearman correlation analysis.Results The composition and change of dominant bacteria of colonic mucosal tissues and colonic lavage fluid were basically the same in 2 groups.The numbers of Bacteroides,Bacteriodes prevotella,Clostridium in colonic mucosal tissues and colonic lavage fluid,and the numbers of Faecalibacterium prausnitzii in colonic lavage fluid of observation group were significantly lower than those in the control group (colonic mucosal tissues:3.68 ± 0.54 vs.4.34 ± 0.27,4.26 ± 0.28 vs.5.33 ± 0.42,4.46 ± 0.68 vs.4.99 ± 0.61;colonic lavage fluid:3.26 ± 0.61 vs.4.09 ± 0.51,3.10 ± 1.42 vs.4.86 ± 1.03,5.24 ± 0.54 vs.5.95 ± 0.51,2.24 ± 1.83 vs.3.24 ± 1.46),and there were statistical differences (P ＜ 0.01 or ＜ 0.05).There was no statistical differences in other dominant bacteria in colonic mucosal tissues and colonic lavage fluid between 2 groups (P ＞ 0.05).Spearman correlation analysisresult showed that the Bifidobacterium,Firmicutes,Faecalibacterium prausnitzii,Lactobacillus colonic mucosal tissues were positively correlated with colonic lavage fluid (r =0.427,0.689,0.577 and 0.369;P =0.041,0.013,0.024 and 0.035),and Enteropathogenic Escherichia coli and Salmonella were negatively correlated with colonic lavage fluid (r =-0.352 and-0.649,P =0.026 and 0.013).Conclusions The composition of dominant bacteria in colonic mucosal tissues and colonic lavage fluid is basically the same,and an imbalance of the microbiota in colonic lavage fluid is found in diarrheapredominant IBS patients.The numbers of Bacteroides,Bacteriodes prevotella,Clostridium and Faecalibacterium prausnitzii significantly decrease.
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Objective@#To investigate the characteristics of dominant bacteria of colonic lavage fluid in patients with diarrhea-predominant irritable bowel syndrome (IBS).@*Methods@#Forty-six patients with diarrhea-predominant IBS (observation group) and 46 patients with abdominal pain or discomfort who was excluded IBS (control group) from January 2016 to January 2019 in Department of Gastroenterology, Shenzhen Longhua District People′s Hospital were selected. During colonoscopy, 2 pieces of descending colonic mucosal tissues were taken by forceps, then 4 ml of colonic lavage fluid was collected from the mucosa tissues to extract total DNA, and 10 dominant bacteria were detected by real-time fluorescence quantitative polymerase chain reaction (including Bacteroides, Bacteriodes prevotella, Bifidobacterium, Clostridium, Enteropathogenic Escherichia coli, Dialister pneumosintes, Firmicutes, Faecalibacterium prausnitzii, Salmonella, Lactobacillus, and data were selected for logarithm). The correlation of dominant bacteria between colonic mucosal tissues and colonic lavage fluid was analyzed by Spearman correlation analysis.@*Results@#The composition and change of dominant bacteria of colonic mucosal tissues and colonic lavage fluid were basically the same in 2 groups. The numbers of Bacteroides, Bacteriodes prevotella, Clostridium in colonic mucosal tissues and colonic lavage fluid, and the numbers of Faecalibacterium prausnitzii in colonic lavage fluid of observation group were significantly lower than those in the control group (colonic mucosal tissues: 3.68 ± 0.54 vs. 4.34 ± 0.27, 4.26 ± 0.28 vs. 5.33 ± 0.42, 4.46 ± 0.68 vs. 4.99 ± 0.61; colonic lavage fluid: 3.26 ± 0.61 vs. 4.09 ± 0.51, 3.10 ± 1.42 vs. 4.86 ± 1.03, 5.24 ± 0.54 vs. 5.95 ± 0.51, 2.24 ± 1.83 vs. 3.24 ± 1.46), and there were statistical differences (P<0.01 or <0.05). There was no statistical differences in other dominant bacteria in colonic mucosal tissues and colonic lavage fluid between 2 groups (P>0.05). Spearman correlation analysis result showed that the Bifidobacterium, Firmicutes, Faecalibacterium prausnitzii, Lactobacillus colonic mucosal tissues were positively correlated with colonic lavage fluid (r = 0.427, 0.689, 0.577 and 0.369; P = 0.041, 0.013, 0.024 and 0.035), and Enteropathogenic Escherichia coli and Salmonella were negatively correlated with colonic lavage fluid (r = -0.352 and -0.649, P = 0.026 and 0.013).@*Conclusions@#The composition of dominant bacteria in colonic mucosal tissues and colonic lavage fluid is basically the same, and an imbalance of the microbiota in colonic lavage fluid is found in diarrhea-predominant IBS patients. The numbers of Bacteroides, Bacteriodes prevotella, Clostridium and Faecalibacterium prausnitzii significantly decrease.
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Objective To investigate the expression level of microRNA-21,microRNA-135b and microRNA-141 in colon cancer tissue.Methods Totally 40 cases of colon cancer specimen from surgical resection in our hospital from May 2013 to April 2014 were selected,40 healthy colon tissues from people who entered our hospital at the same period for physical examination were selected.The expression level of microRNA-21,microRNA-135b and microRNA-141 were determined using real time PCR.Chosed sex,age,lymph node metastasis,TNM staging,differentiation degree as related factors by literatures published in home and abroad,assessed the correlation between the level of microRNA-141,microRNA-135b,microRNA-21 and its expression in colorectal cancer.Results The expression level of microRNA-21,microRNA-135b and microRNA-141 in colon cancer tissues were remarkably higher than those in healthy tissues,the difference was statistically significant(P<0.05).The expression levels of microRNA-141,microRNA-135b and microRNA-21 in colon cancer tissues were closely related to the occurrence of lymph node metastasis,progression and stage of colon cancer,and the difference was statistically significant(P<0.05).Conclusion The expression of microRNA-21,microRNA-135b and microRNA-141 are upregulated in colon cancer tissue,which is highly correlated with the lymphatic metastasis and progressiveness of colon cancer.
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Objective To compare the treatment effects of Qingyi Huoxue decoction and infliximab on acute necrosis pancreatitis ( ANP) complicated with MODS in a rat model. Methods 4.5% sodium taurocholate was injected into the pancreatic duct to induce the ANP complicated with MODS model. The ANP rats were randomly divided into 3 groups, ANP group (ANP), Qingyi Huoxue decoction treatment group ( QG) , infliximab treatment group (IG). Rats in infliximab group received infliximab injection at a dose of 8 mg/kg body weight via tail vein 6 h after the ANP induction. The ANP and QG received normal saline and Qingyi Huoxue decoction (20 ml/kg) via gastric lavage 4 h before and 3 h, 9 h after ANP induction. After 24 h, all rats were sacrificed, the serum levels of amylase, total bilirubin, Cr, TNF-α, diamine oxidase ( DAO) , intra-abdominal pressure (IAP) and the rate of carbon propelling rate in ileum were measured. The pancreas samples were collected for pathological examination. The pathological score of pancreas was calculated. Results The pathological scores in ANP, QG, IG were 13.8 ±0.8, 6.1 ±0.4, 3.9 ±0.6, and the difference was statistically significant (P <0.05). The serum levels of amylase, total bilirubin, Cr, TNF-α were significantly decreased. In ANP, QG, IG the serum levels of DAO were (186.3 ± 10.2 ) , ( 134.6 ± 14.3 ) , ( 149.1 ± 16.3) U/L; the carbon propelling rates in ileum were (53 ±0.1)% , (89 ±0.1)% , (61 ±0.1)% ; the IAPs were (11.8 ±1.5), (4.1±0.8), (5.8 ±1.2) mmHg(1 mmHg=0.133 kPa). The DAO and IAP in AG, IG were significantly decreased when compared with that in ANP group, but the carbon propelling rates in ileum was significantly higher than that in ANP group, and the difference was statistically significant ( P < 0.05). In addition, the carbon propelling rates in ileum in QG were higher than that in IG, and IAP and DAO levels were lower than that in IG, and the difference was statistically significant (P <0.05). Conclusions The Qingyi Huoxue decoction and infliximab were significantly effective in the treatment of ANP rats complicated with MODS. But the effects of Qinyi Huoxue decoction on promoting gastrointestinal motility, reducing the IAP and improving the intestinal barrier function were better than those of the infliximab.
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Objective To investigate the time-course change of intra-abdominal pressure (IAP), TNF-α in rat with acute necrotizing pancreatitis (ANP) and its mechanism. Methods 80 SD rats were randomly divided into ANP group and control group. ANP group was induced by retrograde injection of 4.5% sodium taurocholate into the pancreatic duct in SD rats, while control group was induced by injection of saline at the same dosage. Eight rats in each group were killed at 1 h, 3 h, 6 h, 12 h and 24 h, respectively. The serum levels of amylase, TNF-α were determined;IAP, amount of abdominal ascites, pancreatic pathologic changes were evaluated. Results The levels of amylase in ANP group increased progressively with time, which were 32-folds higher than those in control group at 24 h. The serum levels of TNF-α in ANP group reached the peak at 6 h, and were significantly higher than those in the control group. The amount of abdominal ascites increased post-ANP induction and reached the peak at 24 h, which was 4.7-folds higher than that in the control group. The IAP significantly increased 1 h after induction, which was 3 times as high as the control group in 3 h group and 9 times in 12 h group. Pathologic injuries deteriorated progressively and reached the peak at 24 h. Conclusions Significant IAP occurred in ANP rats and reached the peak at 12 h, TNF-α reached the peak at 6 h, therefore IAP may be the secondary response to inflammatory reaction.
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Objective To explore the dose-effect relationship and optimal concentration of sodium taurocholate (NaTc) to establish acute necrosis pancreatitis (ANP) complicating MODS in a rat. Methods 280 SD rats were randomly divided into control group (n =40) and 3.5%, 4%, 4.5%, 5% ANP group (n = 60, respectively). ANP groups were induced by retrograde injection of NaTc solution into the pancreatic duct of rats. Rats in each model group were sacrificed at 1, 3, 6, 12 and 24 h after the model induction. Serum amylase, serum total bilirubin, creatinine, blood gas analysis, and pathological changes of pancreas were determined. Survival rate at 24 h after the model induction was observed in other 20 ANP rats in each group. Results In each groups, the serum amylase level, creatinine, blood gas analysis and pathological changes all increased corresponding to the increase of NaTc, and there was significant dose-effect relationship and time-effect. In 4.5% group, the dysfunction of liver and kidney reached the peak at 6 h,while the respiratory dysfunction reached its peak at 12 h. Its incidence of MODS was 52.5%, which was significantly higher than those in 3.5%, 4.0% group (15.0% and 37.5%) ;and 24 h survival rate of 4.5% group was 65%, which was significantly higher than that in 5% group (30%). Conclusions 4.5% NaTc solution may be the optimal concentration to establish the ANP model, which had higher incidence of MODS and 24 h survival rate.